The initiator tRNA genes fromStreptomyces rimosus
نویسندگان
چکیده
منابع مشابه
How many initiator tRNA genes does Escherichia coli need?
Multiple copies of a gene require enhanced investment on the part of the cell and, as such, call for an explanation. The observation that Escherichia coli has four copies of initiator tRNA (tRNAi) genes, encoding a special tRNA (tRNA(fMet)) required to start protein synthesis, is puzzling particularly because the cell appears to be unaffected by the removal of one copy. However, the fitness of ...
متن کاملFrom elongator tRNA to initiator tRNA.
We show that the two most important properties needed for a tRNA to function in initiation in Escherichia coli are its ability to be formylated and its ability to bind to the ribosomal P site. This conclusion is based on conversion of two different elongator tRNAs to ones that can act as initiators in E. coli. We transplanted the features unique to E. coli and eubacterial initiator tRNAs to E. ...
متن کاملReducing the fitness cost of antibiotic resistance by amplification of initiator tRNA genes.
Deformylase inhibitors belong to a novel antibiotic class that targets peptide deformylase, a bacterial enzyme that removes the formyl group from N-terminal methionine in nascent polypeptides. Using the bacterium Salmonella enterica, we isolated mutants with resistance toward the peptide deformylase inhibitor actinonin. Resistance mutations were identified in two genes that are required for the...
متن کاملMore surprises in translation: initiation without the initiator tRNA.
O of the most widespread beliefs in molecular biology is that protein synthesis is initiated with methionine or formylmethionine in all organisms, by using AUG as the initiation codon and a special methionine tRNA called the initiator tRNA (1, 2). Eubacteria, mitochondria, and chloroplasts initiate protein synthesis with formylmethionine, whereas archaebacteria and eukaryotic cytoplasm initiate...
متن کاملinitiator tRNA analogs with different nucleotides in the
The ef fec t of base changes a t the fourth pos i t ion from the 3 ' terminus of Esoheriohia c o l i i n i t i a t o r tRNA has been s tudied to t e s t the ' d i s criminator hypothesis ' which proposed tha t the nucleot ide in t h i s pos i t ion might have a r o l e i n the s p e c i f i c i t y of the aminoacylation r eac t ion . £ . ool i i n i t i a t o r tRNA lacking the 3 ' t e rmina l ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.16.6747